Multiphoton Fluorescence and Phosphorescence Imaging (FLIM/PLIM) Microscopes

The techniques employed on this instrument are

Technical specifications

Instrument Nikon EC1, EC2, Becker and Hickl DCS120 and custom systems 
Laser wavelengths Femtosecond Chameleon Discovery NX Lasers, 660 to 1330nm, Coherent APE OPO 505 to 640nm, pulse selection unit 

One-photon laser, Diode laser, NKT super continuum 405 to 2,000nm 

Compatible objective lenses 10x, 40x air, 20x NA 0.75, 63x oil immersion, 60x water immersion NA 1.27, 60x air ELWD, 100x oil immersion NA 1.49 
Compatible dyes Any, customable filter. 
Compatible sample mounts Cover slip on microscope slide or glass bottom Petri dish 
Configuration Inverted microscope 
Temperature control Environmental chamber or heated stage insert, 5 to 50°C. gases include N2, CO2, O2, Ar or any mixture 
Lateral resolution ~350nm (depends on wavelength) 
Axial resolution ~800nm (depends on wavelength) 
Temporal resolution From 25ps (combined with FLIM) 

 

Relevant contacts

Stanley Botchway

UKRI-STFC Fellow and Research Lead

Stan joined the CLF in 1998 as the Laser Microscopy and Imaging Facility Manager and Biosciences Lead following a Postdoctoral fellowship at Harvard Medical School.