Leica SP8 tauSTED

The techniques employed on this instrument is Raster Image Correlation Spectroscopy,

Technical specifications

Objectives lenses  HC PL APO CS 10x/0.40 DRY, HC PL APO CS2 63x/1.4 OIL, HC PL APO 63x/1.20 W CORR CS2, HC PL APO 100x/1.40 OIL STED WHITE, HC PL APO 86x/1.20 W motorised correction STED WHITE 
Lasers  405 Diode, supercontinuum WLL (470 to 670nm, 8 simultaneous laser lines, 80MHz, with pulse picker), STED: CW 592nm, CW 660nm, pulsed 775nm 
Detectors  Photon counting hybrid GaAsP-Avalanche Photodiode (HyD) x3 

Photomultiplier tube (PMT) and one transmitted light detector x2 

Environmental 

control 

Okolab stage top incubator temperature, CO2 mixer and humidity 

 

Software 

licences 

LAS X (Live Data Mode, FRAP, FRET, FCS, HyVolution), Huygens Everywhere