Leica SP8 tauSTED

The techniques employed on this instrument is Raster Image Correlation Spectroscopy,

Technical specifications

Objectives lenses HC PL APO CS 10x/0.40 DRY, HC PL APO CS2 63x/1.4 OIL, HC PL APO 63x/1.20 W CORR CS2, HC PL APO 100x/1.40 OIL STED WHITE, HC PL APO 86x/1.20 W motorised correction STED WHITE 
Lasers 405 Diode, supercontinuum WLL (470 to 670nm, 8 simultaneous laser lines, 80MHz, with pulse picker), STED: CW 592nm, CW 660nm, pulsed 775nm 
Detectors Photon counting hybrid GaAsP-Avalanche Photodiode (HyD) x3 

Photomultiplier tube (PMT) and one transmitted light detector x2 

Environmental 

control 

Okolab stage top incubator temperature, CO2 mixer and humidity 

 

Software 

licences 

LAS X (Live Data Mode, FRAP, FRET, FCS, HyVolution), Huygens Everywhere 

 

Relevant contacts

Esther Garcia Gonzalez

Microscopy Link Scientist

Esther joined the CLF in 2022. She leads the user applications for the Stimulated Emission Depletion (STED) microscope.

Kirstie Davies

Octopus Industrial Placement