Experiment information
The MONSTER microscope is a combined ZEISS LSM880-Elyra PS1 system, in which simultaneous multicolour imaging is enabled thanks to the dual EMCCD camera setting. It incorporates multiple imaging modalities, shown below.
Technical specifications
| Confocal | Airyscan | SIM | SMLM (PALM/STORM) |
|
| Laser wavelengths (nm) | 405, 458, 488, 514, 561, 633 | 405, 458, 488, 514, 561, 633 | 405, 488, 561, 642 | 405, 488, 561, 642 |
| Simultaneous dual colour imaging | Yes |
No | Yes | Yes |
| Compatible objective lenses | C-Apo 40x/1.20 w, Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil) | C-Apo 40x/1.20 w, Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil) | Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil) | Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil) |
| Field of View (μm) | 135×135 (63x/1.40 NA objective) | 135×135 (63x/1.40 NA objective, Fast mode) | 80×80 (63x/1.40 NA objective) | 14×14 (100x/1.46 NA objective, µHP mode)/26×26 (HP mode) |
| Lateral resolution (nm) (λ=488nm) | 240 (63x/1.40 NA objective) | 140(normal mode)/120(2D SR or 3D mode)/145 in X, 180 in Y (Fast SR mode) | 120 | 20 (localisation precision) |
| Axial resolution (nm) (λ=488nm) | 700 (63x/1.40 NA objective) | 350 (normal mode)/ 450 (Fast SR mode) | 300 | 50 |
| One shot 3D method | N/A | N/A | N/A | phase ramp, 1µm depth |
| Sample thickness (μm) | 100 | 100 | 20 | 10 |
| Temporal resolution | 0.85fps at 480×480 pixels | 27fps at 480×480 pixels in Fast mode | 1.6 second per frame (at 24ms integration time) | several minutes |